Arthropod repelling constituents from a southern folk remedy: Investigations of the American beautyberry, Callicarpa americana

AGRO 199

Charles L. Cantrell, Charles.Cantrell@ARS.USDA.GOV1, Charles T. Bryson, cbryson@ars.usda.gov2, Stephen O. Duke, sduke@olemiss.edu1, Jerome A. Klun, klunj@ba.ars.usda.gov3, and John F. Carroll, john.carroll@ars.usda.gov4. (1) Natural Products Utilization Research Unit, USDA-Agricultural Research Service, P.O. Box 8048, University, MS 38677, (2) Southern Weed Science Research Unit, USDA-Agricultural Research Service, P.O. Box 350, Stoneville, MS 38776, (3) Chemicals Affecting Insect Behavior Laboratory, USDA-Agricultural Research Service, Beltsville Agricultural Research Center, 10300 Baltimore Ave., Bldg. 007, Rm. 301, BARC-West, Beltsville, MD 20705, (4) Animal Parasitic Diseases Laboratory, USDA-Agricultural Research Service, Beltsville Agricultural Research Center, Bldg. 1040, BARC-East, Beltsville, MD 20705
Based on botanical lore of insect repellent properties, essential oil extracts from Callicarpa americana and Callicarpa japonica were investigated. Bioassay-guided fractionation of C. americana extracts using the yellow fever mosquito, Aedes aegypti, led to the isolation of α-humulene, humulene epoxide II, and intermedeol, and a newly isolated terpenoid (callicarpenal). Similar work involving C. japonica resulted in the isolation of an additional compound, spathulenol, as well as the four compounds isolated from C. americana. Heretofore, 13,14,15,16-tetranor-3-cleroden-12-al, callicarpenal, has never been identified from natural sources. In bite-deterrent studies spathulenol, intermedeol, and callicarpenal showed significant bite-deterring activity against Aedes aegypti and Anopheles stephensi. The repellency of callicarpenal and intermedeol against workers of red imported fire ants, Solenopsis invicta and black imported fire ants, Solenopsis richteri will also be reported. In addition, callicarpenal and intermedeol were evaluated in laboratory bioassays for repellent activity against host-seeking nymphs of the blacklegged tick, Ixodes scapularis, and lone star tick, Amblyomma americanum and results will be presented.
 

Agricultural Biomass, Biobased Products, and Biofuels
8:30 AM-12:10 PM, Thursday, March 29, 2007 McCormick Place South -- Room S103D, Level 1, Oral

Division of Agrochemicals

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007