CARB 114 |
| The fermentation profile of sugar beet pulp and pectic oligosaccharides (POS) derived from sugar beet and Valencia oranges were determined in vitro. The Valencia orange POS consisted of the arabinogalactan pectic side chains and xyloglucan. However, the sugar beet POS consistsed of homogalacturonan oligosaccharides, rhamnogalacturonan I and II as well as the arabinogalactan pectic side chains and minor amounts of xyloglucan. Temperature and pH controlled, stirred anaerobic fecal batch cultures were used and fermentation end products and bacterial population changes were determined. POS from Valencia oranges was found to be bifidogenic after 24 hours fermentation and all novel test substrates caused an increase in acetate, butyrate and propionate concentrations. POS substrates were investigated for their ability to neutralise Shiga-like toxins from Escherichia coli O157:H7 and POS from Valencia oranges was able to neutralise the effects of the toxins at 10µgml-1. During this study, POS from sugar beet was found to inhibit the growth of the colon cancer cell line HT29 and on further investigation a dose response was found. This finding may have implications for dietary protection against colon cancer. |
|
Biological Applications of Carbohydrates
8:30 AM-12:10 PM, Thursday, March 29, 2007 McCormick Place North -- Room N226, Level 2, Oral
Division of Carbohydrate Chemistry |