CHED 301 |
| In accordance with the Stern-Volmer equation, dynamic quenching of fluorescence spectra involves the non-radiative deexcitation of a fluorophore through collisions with a quencher (often dissolved oxygen) during the fluorophore's lifetime. In order to avoid problems associated with the quantitative analysis of obtained fluorescence data in the presence of dynamic quenching by oxygen, a method of purging solutions with an inert gas is typically applied. Purging methods remove dissolved oxygen from solutions in accordance with Henry's Law. This kinetic investigation focuses on the purging of anthracene (and related compounds) in cyclohexane with nitrogen gas. Steady-state fluorescence, absorbance, and fluorescence lifetime data are simultaneously collected throughout the study with a novel fiber-optic based instrumental set-up. Optimal purging conditions for the deoxygenation of solutions are described for the systems studied. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |