CHED 298 |
| The purpose of this research is to develop an entangled polymer solution for short tandem repeat (STR) separations on microfluidic devices. STRs are genomic elements consisting of 2-7 basepair sequences repeated various number of times. Recently a number of commercial systems have appeared using short fluidic channels for the rapid detection of native PCR amplified DNA using laser induced fluorescence. To be applicable for STR analysis these systems require improved separation efficiency and /or redesign due to their shorter separation channels. Currently, the resolution for the commercial DNA chip systems is approximately 5 bp. To improve resolution we are examining the application of polymeric buffers in combination with denaturants. The buffer utilized in this research includes Polyvinylpyrrolidone (PVP) (MW= 1,000,000 g/mol) in Tris-Borate-EDTA (TBE) buffer, with a urea denaturant for single stranded DNA analysis. Initial results in standard CE systems show good reproducibility for this polymer with minimal osmotic flow. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |