CHED 918 |
| This research is designed to examine the role of a component within the membrane domain of the yeast V-ATPase. Through computational and experimental analysis, a sequence of amino acids was deleted from the gene that codes for the subunit being investigated, subunit d. Amino acids two through seventy-five were deleted from the gene VMA6. Based on the results and visual models obtained, it is obvious that this large deletion will have a dramatic effect on the overall assembly and function of the yeast V-ATPase enzyme. The deletion performed may hinder the ability of the V0 sector to form within the membrane, which would lead to the catalytic domain, V1, not binding to the membrane domain to form the V1V0 complex. Since the function of the V-ATPase is to control the internal acidic pH of a cell or organelle, the absence of the V1V0 complex would lead to an increase in the pH within the cellular system, resulting in the death of the cell. |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |