CHED 1126 |
| Pyoverdines are yellow-green, water soluble, fluorescent pigments that are produced by Pseudomonas spp. bacteria. They are complex peptidic siderophores, acting as strong FeIII chelators and allowing Pseudomonads to scavenge iron from limited sources. This project focuses on isolation and purification of pyoverdines produced by Psuedomonas putida from soils in Ventura, California, and the characterization of metal complexes of the purified pyoverdines. Pyoverdines were isolated from bacterial cultures by solid-phase extraction and purified by ion-exchange and size-exclusion chromatography. Purity was analyzed by HPLC and siderotyping was undertaken by LC-MS. The ground state and excited state photophysical properties were examined for both the free pyoverdine and pyoverdine-metal complexes with V, Cr, Mn, Fe, Co, and Ni. |
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Undergraduate Research Poster Session: Environmental Chemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |