CHED 458 |
| Photonic Explorers for Biomedical use by Biologically Localized Embedding (PEBBLEs) containing the Mg2+ sensing fluorophore Coumarin 343 were introduced into the model organism Dictyostelium discoideum to detect fluxes of intracellular magnesium ion concentration during chemotaxis. Unlike most magnesium sensing dyes, Coumarin 343 is highly selective for magnesium, and when immobilized within a PEBBLE is nontoxic to organisms1. In contrast, fluorescent dyes from Molecular ProbesTM have a greater affinity for Ca2+ than Mg2+ 2. Quantum dots, also immobilized in the PEBBLEs, served as a reference signal to which changes in the intensity of the Coumarin 343 peak were compared. Calibrated PEBBLEs were successfully introduced into Dictyostelium discoideum amoeba via engulfment, and the fluorescence of the Coumarin 343 PEBBLEs was monitored on a cellular basis during cAMP stimulation. Coumarin 343 PEBBLEs offer a selective and noninvasive procedure for monitoring changes in cytosolic Mg2+ concentration. (1) Park, E. J.; Brasuel, M.; Behrend, C.; Philbert, M. A.; Kopelman, R. Analytical Chemistry 2003, 75, 3784-3791. (2) Molecular Probes Catalogue, 2005.
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Undergraduate Research Poster Session: Nanotechnology
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |