Analysis of volatile compounds of diagnostic interest using a microdialysis probe extraction technique

CHED 270

Ashley M Kramer, amkramer@stthomas.edu and Anthony Borgerding, ajborgerding@stthomas.edu. Department of Chemistry, University of Saint Thomas, 2115 Summit Ave, Saint Paul, MN 55105
Our lab has successfully used microdialysis probes for volatile compound extraction from solutions into the gas phase as opposed to extraction into liquid dialysis. In our research volatile compounds were extracted from aqueous solutions through microdialysis probes using gas chromatography with a flame ionization detector. Compounds include acetone and dimethyl sulfide, showing a linear representation over a range of concentration (17.2-1720mM and 10-200mM, respectively). Recently, volatile compounds that are insoluble in water have been analyzed from organic solvent solutions using microdialysis probes interfaced to a mass spectrometer. Results were obtained for carbon disulfide over a range of 10-200mM using this method. Isoprene and halothane will also be tested. Our research lab is currently testing our probes on these volatile compounds (found in human breath condensate) for the long-term goal of using microdialysis as an in-vivo method of study in biochemical systems such as different places in the human body.