CHED 882 |
| Largemouth bass, (Micropterus salmoides), are extremely important as a recreational sportfish and are becoming an important candidate for freshwater fish farming. When a fish farm in West Barnstable, MA experienced increased bass mortalities, diagnoses indicated a unique and understudied monogenetic trematode, Acolpenteron ureterocetes, as the primary cause of fish mortality. The need for an accessible way to eradicate, or at least control, A. ureterocetes is critical. This research focused on the effects of a commercial anti-trematode treatment, Praziquantel, administered under controlled conditions. A necropsy of the kidney provided histopathological slides for comparison before and after parasite exposure and treatment. The body burden of Praziquantel in the bass kidney was determined by fluorescence spectroscopy (λex=260nm and λem=285nm, 100ng detection limit). This data was compared with the concentration of the chemical remaining in the water column to yield a mass balance. UV/Vis spectroscopy was used to determine the environmental degradation rate of Praziquantel in water. The derivatives of Praziquantel were determined with HPLC/MS to elucidate whether the parent compound was intact or found in the tissue as a metabolite. |
|
Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |