Exploring and exploiting self assembly with DNA nanotubes

CHED 842

Deborah Kuchnir Fygenson, deborah@physics.ucsb.edu1, Kyle Chipman2, Kim Weirich2, and Patrick O'Neill1. (1) Physics Department, UC Santa Barbara, Santa Barbara, CA 93106, (2) Biomolecular Science & Engineering Program, UC Santa Barbara, Santa Barbara, CA 93106
Short sequences of DNA can be designed to self-assemble into extended structures based on Watson-Crick pairing rules. The most generic design scheme makes use of building blocks, or “tiles”, of three or more strands that hybridize into a core of cross-linked double helices with single-stranded sticky ends. We use tiles known as double-crossovers (DX units) to self-assemble tubular polymers of DNA that are up to 100 µm in length, ~10 nm in diameter and correspondingly stiff. We seek to understand how tile design determines the kinetics and thermodynamics of nanotube nucleation and growth and so lay a foundation for practical applications of this and other tile-based nucleic acid architectures. Applications include reproducing biological phenomena (e.g. microtubule dynamic instability) and replacing sub-micron lithographic patterning.
 

Exploring and Exploiting Nature with Biomimetics
1:30 PM-4:45 PM, Monday, March 26, 2007 McCormick Place North -- Room N230A, Level 2, Oral

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007