CHED 1208 |
| Guanine is a constituent of nucleic acids and as such, guanine-metal ion interactions bear significant biochemical importance. This study was designed to examine the interaction of guanine with four metal ions (cadmium, lead, mercury, and zinc). The study was carried out at different pH values and metal ion/ligand concentrations. Infrared and Raman spectroscopes were used to determine whether coordination occurs through the amino group or carbonyl group, or both. Upon complexation, the infrared and Raman spectra of guanine at low pH showed a shift in frequency and a decrease in intensity of the C=O band, suggesting that the coordination of metal ions in guanine occurs through the O atom of the C=O group at acidic pH. At higher pH, only mercury and zinc were found to interact with guanine. The relative stability constants for the binding of guanine by metal ions were determined. The tautomerism of guanine was also discussed. |
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Undergraduate Research Poster Session: Inorganic Chemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |