CHED 932 |
| Polyunsaturated carbon chains are the most effected by oxidation, which is a danger to human health. The goals of these experiments were to identify and separate oxidized fatty acids on the HPLC and GC, and test the effects of oxidized fatty acids on zinc transport. Unoxidized egg phosphatidylcholine (PC) were separated on HPLC and, after transesterification, the fatty acid methyl esters were identified on GC. As the separation of oxidized species was not complete, a C-18 polyvinylalcohol column was substituted for the standard C-18 silica HPLC column. There are a variety of oxidation products that can form; the primary products are a set of isomers (hydroperoxy and conjugated diemers). To simplify the HPLC chromatogram and test the ability of the GC to separate primary oxidation product isomers, linoleate methyl ester was oxidized. The oxidized linoleate methyl ester was separated on the HPLC and then GC was used to identify the peaks obtained from the HPLC. A PUA column was used to improve separation of oxidized linoleate methyl, which has a higher resolution. |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |