A major problem encountered with the performance of biofuel cells is the leaching of electronic mediator species from immobilizing polymer membranes coating modified anodes and/or cathodes. Where enzyme-electrode communication depends on mediated electron transfer, leaching of the mediator into the biofuel cell electrolyte can significantly diminish the running time of the device. Using standard methodology, we have synthesized the complex [Ru(k²-2,2′-N₂C₁₀H₆{(CH₂)₃(CF₂)₅CF₃}₂-4,4′)(k²-2,2′-N₂C₁₀H₁₀)₂], where one of the 2,2′-bipyridyl ligands bears terminal perfluorinated alkyl chains, in attempt to exploit ‘like-dissolves-like' intermolecular forces as an anchoring mechanism for the complex in Nafion membranes. Nafion thin films were cast or spin-coated onto electrodes and quartz cuvettes, exposed to MeCN solutions of the complex and then examined by cyclic voltammetry and fluorimetry in a comparative study with tris-2,2′-bipyridyl ruthenium.