CHED 275 |
| Methods used for the diagnosis of excessive alcohol consumption are based on indirect alcohol markers such as erythrocyte mean cell volume (MCV) or carbohydrate-deficient transferring, with the disadvantage that increased values can also originate from other phatological conditions. Fatty acid ethyl esters are products of nonoxidative ethanol metabolism. Recently Volker Auwärter and coworkers showed that FAEE hair concentrations can be used as markers for excessive alcohol consumption. They used ethyl myristate, ethyl palmitate, ethyl oleate and ethyl stearate as markers. Since FAEEs are deposited on hair mainly from sebum111, and hair analysis is complicated, the viability of the MAE-SPME-GC/MS method for the detection of FAEES is being evaluated. A qualitative analysis was done in which the presence of ethyl myristate, ethyl palmitate, ethyl oleate and ethyl stearate were evaluated. From the analysis it was noticed that number of identified FAEEs increased with an increase in alcohol consumption This preliminary study established the viability of the detection of FAEEs in sebum with the developed methodology. A quantitative analysis with more participants needs to be performed to establish if the concentration of FAEEs allow discrimination between different categories of alcohol consumption (e.g. social drinkers, alcoholics) |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |