Surface-enhanced fluorescence

CHED 408

Arika Pravitasari1, Tanya Shtoyko, tshtoyko@mail.uttyl.edu1, Evgenia G. Matveeva, ematveev@hsc.unt.edu2, Ignacy Gryczynski3, and Zygmunt Gryczynski, zgryczyn@hsc.unt.edu2. (1) Department of Chemistry, The University of Texas at Tyler, 3900 University Blvd., Tyler, TX 75799, (2) Department of Molecular Biology and Immunology, University of North Texas, Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, (3) Department of Cell Biology and Genetics, University of North Texas, Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107
Among optical instrumental techniques, fluorescence spectroscopy is recognized as one of the more sensitive. We have designed a laboratory experiment to illustrate the use of surface–enhanced fluorescence for determination of Rhodamine Red on silver nanoparticles. This experiment is highly applicable to an undergraduate laboratory course in Analytical Chemistry. Students will be asked to synthesize silver nanoparticles and deposit them on glass slide. Rhodamine-Red will be attached to the silver nanoparticles on the glass slide and bare glass slides. The fluorescence emission due to Rhodamine-Red dye will be measured and compared to the fluorescence emission of the dye on the bare glass slide. The fluorescence enhancement will be calculated. Surface- enhanced fluorescence has a great potential application in chemical and biomedical sensing.
 

Undergraduate Research Poster Session: Chemical Education
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007