CHED 263 |
| We have established Dictyostelium discoideum as a model for testing nano-optical ion sensors. The field of nano-optical sensors is growing rapidly but there are few established protocols for technology transfer to fields such as biology. In recent years, PEBBLEs (Photonic Explorers for Biomedical use by Biologically Localized Embedding) have been demonstrated in a broad range of applications, but a common platform for intracellular response characterization has not been established. The slime-mold, Dictyostelium discoideum , is developed as such a platform, and will be established as a rapid screen for ion-sensing particles. Current efforts are to quantify Ca2+ fluxes in response to cAMP, which the cell uses as an aggregation signal. This will allow the comparison of the response of PEBBLE Ca2+ sensors to accepted methods of measurement. Our ability to quantify these intracellular responses will give further insight into the role of ion channels in biological processes. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |