CHED 931 |
| Fifty million people a year suffer from amoebiasis, caused by the intestinal protozoan Entamoeba histolytica. Studies show that the iron active center of the ADH domain in the EhADH2 enzyme from E. histolytica is essential for fermentative growth. This study demonstrates the importance of iron (II) in anaerobic growth of transformed E. coli cells and the biochemical activity of EhADH2. Transformed E. coli cells were grown under optimal anaerobic growth conditions. The optimal medial growth conditions were altered in increased ferrous (II), in zinc (II) (a competitive inhibitor), or in 1, 10- phenanthroline (a metal chelator). The E. coli cells grown under the various conditions were lysed by French press, and the enzyme was purified by FPLC. The isolated enzyme concentration was determined using the Bradford Assay. Activity of the EhADH2 enzyme was determined with substrate kinetics using acetaldehyde as a substrate and monitored by UV/Vis (340 nm). NIH-NCRR Grant #2P20RR1645704 |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |