Effect of copolyether-urethane-urea domain-matrix morphology on protein adsorption

CHED 791

Jessica Moseley, mosjes03@evergreen.edu1, Bronwyn Bacon1, Ryan D. Bonifield1, Marianne Moore1, Jade Rivera1, Donald J. Lyman2, and Paula Schofield1. (1) The Evergreen State College, Lab I, 2700 Evergreen Parkway NW, Olympia, WA 98505, (2) Dept. of Bioengineering and Dept. of Materials Science and Engineering, University of Utah, Salt Lake City, UT
The micro-phase separation of block copolyether-urethane-ureas (PEUU's) into a domain-matrix morphology appears to contribute to their biocompatibility. The purpose of this study was to synthesize and compare the biocompatibility of a standard synthesis PEUU with a PEUU having more-pure block sizes prepared by a multi-step synthesis. Although the FT-NMR spectra of the two PEUU's were similar, the ATR-FTIR spectra of the pure PEUU showed an increase in the hydrogen bonded amide I (1710 cm-1) relative to the free carbonyl (1730 cm-1), indicating improved phase separation due to more-pure block sizes. Biocompatibility was determined from the composition of the adsorbed protein layer formed by exposing PEUU films to a solution of albumin, gamma globulin, and fibrinogen. While Bradford and Hartree-Lowry protein assays and diode array analysis were unreliable in determining the relative composition, SDS-PAGE and ATR-FTIR analyses appear to be more effective in determining the adsorbed protein layer composition.
 

Undergraduate Research Poster Session: Polymer Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007