CHED 296 |
| A protocol was developed to measure the association constant (KS) of a small molecule fluorophore and a non-fluorescent binding partner. Fluorescence intensity data was collected during titrations of the fluorescent ligands and association hosts, and were fit using non-linear regression analysis to determine the KS of each ligand/binding host pair. The fluorophores 8-anilino-1-naphthalenesulfonate (ANS) and dimethylaminonaphthalene sulfonyl (dansyl) amide were used as small molecule ligands because they exhibit enhanced fluorescence and a hypsochromic shift in their emission spectra when bound to a macromolecular host. The binding hosts used in the development of this protocol were â-cyclodextrin (BCD), hydroxypropyl-â-cyclodextrin (HPBCD), and an antibody raised against the dansyl fluorophore (anti-DAN). The resulting protocol can be used to determine the KS between ANS or dansyl amide and water soluble macromolecular hosts. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |