ATR-FTIR analysis of the interaction of biotinylated dendrimers with blood brain barrier models

CHED 455

Jerome D Hogan, hoganj1@nku.edu1, Thomas Fabre, fabret@nku.edu1, Keith A. Walters, walterske@nku.edu1, Kristi L. Haik, martines@nku.edu2, Donald A. Tomalia3, and Heather A. Bullen, bullenh1@nku.edu1. (1) Department of Chemistry, Northern Kentucky University, Nunn Dr, Highland Heights, KY 41099, (2) Department of Biological Sciences, Northern Kentucky University, Nunn Dr, Highland Heights, KY 41099, (3) Dendritic NanoTechnologies, Inc, 2625 Denison Drive, Mount Pleasant, MI 48858
Dendrimers have significant pharmaceutical appeal because their structures provide a possible mechanism for carrying drugs across the blood brain barrier (BBB). The BBB is a tight seal of cells that create a tight junction preventing harmful agents in the bloodstream from reaching the brain . The BBB also prevents the passage of many drugs that target illnesses of the brain. The focus of this research project is the characterization of the interaction of biotinylated G4 and G5 PAMAM dendrimers with model blood brain barriers using attenuated total reflectance infrared spectroscopy (ATR-FTIR). Avidin and biotin have a very high binding affinity for one another and are fairly inexpensive, making them ideal for the use as markers for PAMAM dendrimers. Results on the synthesis and variable angle ATR-FTIR analysis of their interaction with a model BBB, DMPC (Dimyristoyl-sn-Glycero-3-Phosphocholine) liposomes, are presented here.

 

Undergraduate Research Poster Session: Nanotechnology
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007