Electrosynthesis of surface-bound hydroxylamine linkers for antibody immobilization

CHED 306

Boone M. Prentice, bmp595@longwood.edu1, Melissa C. Rhoten, rhotenmc@longwood.edu1, Dwight A Williams, williamsda2@vcu.edu2, Suzanne M. Ruder, sruder@vcu.edu2, and Charlene D. Crawley, cdcrawle@vcu.edu2. (1) Department of Chemistry and Physics, Longwood University, 201 High Street, Farmville, VA 23909, (2) Department of Chemistry, Virginia Commonwealth University, 1001 W. Main St, Richmond, VA 23284
There is an increasing need for fast, accurate, repeatable and economical methods for the analysis of biohazards and environmental toxins in air and water. Immunoassays have traditionally been used to detect and quantify toxins, but suffer from long analysis times, one-time use, automation challenges, and quantification variability. The use of polarized liquid crystal materials for antibody immobilization is a novel approach in immunosensor design. In this work 4-(4-nitro-phenylethynyl)-benzenethiol has been synthesized and allowed to self-assemble at gold quartz crystal microbalance (QCM) electrodes. Prior to covalent attachment of the antibody of interest, the nitro group must be reduced to a hydroxylamine. This is accomplished electrochemically and is the focus of this poster. Results for the electroreduction of the nitro group of 4-nitrothiophenol to the hydroxylamine is also presented to demonstrate the feasibility of this approach. The electroreduction at gold QCM electrodes is also compared to results obtained at molybdenum electrodes.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007