CHED 330 |
| A flow injection analysis system (FIA) was constructed to study electrochemiluminescence (ECL) reactions. This FIA consisted of a homemade flow cell with a Pt working electrode for light generation and a viewing window for collection of photons with a photomultiplier tube. The ECL of a class of antibiotics called fluoroquinolones was studied in order to determine what particular structures within the fluoroquinolones were responsible for ECL. The system was optimized for enrofloxacin (enro). The greatest light intensity was observed when enro was in a solution with 2 mM tris (2,2'-bipyridyl) ruthenium (II) and a pH 6 buffer, with an applied potential of 1.2 V and a flow rate of 1 mL/min. Using these parameters, several other fluoroquinolones were studied. It was determined that a piperazine ring in the structure was necessary for ECL to take place. Additionally, solutions containing 1-methylpiperazine and dimethylpiperazine were analyzed and ECL was observed.
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |