Effects of the genetic background of (Na+, K+)-ATPase, α-2 knockout mice on the distribution of α-2 and α-3 isoforms of this enzyme in the brain

CHED 963

Adrienne M. Baran1, Natalie R. Meyer1, Theresa M. Spranger1, Jerry B. Lingrel2, Amy E. Moseley2, and Mary Lou Caspers, casperml@udmercy.edu1. (1) Department of Chemistry and Biochemistry, University of Detroit Mercy, 4001 W. McNichols, Detroit, MI 48221, (2) Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati Medical School, Cincinnati, OH
[3H]Ouabain was used to study the distribution of the α-2 and α-3 isoforms in five brain regions of adult wild type and heterozygous α-2 knockout mice of C57BL6, 129/Black Swiss and FVBN genetic backgrounds. In 129/Black Swiss and FVBN α-2 heterozygotes, significant decreases in the relative density of [3H]ouabain binding sites were observed in all five brain regions relative to the same brain region of wild type mice. The 129/Black Swiss α-2 heterozygotes showed the greatest decrease (between 16%-29%) in [3H]ouabain binding sites in all five regions, while the FVBN α-2 heterozygotes had decreases of 11%-16%. Small, but significant, decreases in [3H]ouabain binding were noted only in the cerebral cortex (2%), hippocampus (7.5%), and thalamus (4%) of α-2 heterozygous C57BL6 mice relative to wild type animals. Consequently, the genetic background of the mouse affects the distribution of α-2 and α-3 isoforms of the (Na+, K+)-ATPase in several brain regions.

 

Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007