Cytotoxicity of functionalized hexagonal mesoporous silicates in IEC-18 and MDCK cells for their use as non-steroidal anti-inflammatory drug carriers

CHED 960

Daniel J Mayo, dm0769@ship.edu1, Melissa Zastrow2, Isabelle L. Lagadic, Isabelle.Lagadic@uconn.edu2, and Robin L. McCann, rlmcca@ship.edu1. (1) Department of Chemistry, Shippensburg University, 1871 Old Main Drive, Shippensburg, PA 17257, (2) Department of Chemistry, University of Connecticut, 55 N. Eagleville Rd., Unit 3060, Storrs, CT 06269-3060
Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) have a low solubility in water decreasing drug bioavailability. This is typically improved by grinding the drug with excipients ultimately resulting in recrystalization and insolubility of the drug. A proposed solution to this problem is the use of functionalized hexagonal mesoporous silicates (HMS) as nanoreserviors for the controlled release and solubility improvement of NSAIDs. The toxicity of pure HMS materials must be examined prior to their use as oral drug delivery systems. The cytotoxicity of two different HMS materials was determined in cells lines IEC-18 (Rat Ileum Epithelial Cells) and MDCK (Madin Darby Canine Kidney Cells). A non-radioactive cell proliferation assay (CellTiter96 Non-Radioactive Cell Proliferation Assay, Promega) was used to determine the LD50 of pure HMS and of HMS containing amino groups (HMS-NH2) in both cell lines.

 

Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007