CHED 973 |
| RNA triple helices form when a single stranded region of an RNA molecule binds to the major groove of a preformed Watson-Crick-base-paired hairpin, through Hoogsteen interactions. RNA triple helices can be intermolecular or intramolecular. An intermolecular triple helix is formed from two or three different RNA strands while an intramolecular triple helix is formed from a single RNA strand. This study utilizes UV thermal denaturation and difference spectra to investigate the stability difference between intermolecular and intramolecular triple helices consisting of the same sequence of base triples. The effect that increasing the size of the loop connecting the Hoogsteen region to the Watson-Crick hairpin of an intramolecular RNA triple helices has on the stability of the triple helix will also be investigated. In this study the loops are closed with a possible CG base pair. Melting temperatures and thermodynamic parameters values will be presented. |
|
Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |