Improved electrochemical biosensor response via metal oxide pre-oxidation of chemical interferents

CHED 325

Mark A. Tapsak, mtapsak@bloomu.edu and Jamie G. Houseknecht, jghousek@bloomu.edu. Department of Chemistry, Bloomsburg University of Pennsylvania, Bloomsburg University, 400 East Second Street, Bloomsburg, PA 17815
Typical biological samples are inherently complicated. They not only contain the analyte of interest, but myriad compounds that may be electroactive at the potentials useful for electrochemical biosensors. Therefore, steps must be performed in order to either eliminate or block the interferents from generating false positive signals. A biosensor design that exposes a sample to an insoluble compound that oxidizes interferents such as ascorbic acid, acetaminophen and uric acid was investigated in a prototype sensor system at a potential of 0.6 V versus Ag/AgCl. An enabling fabrication technology was implemented to generate structures for this electrochemical measurement application. The unconventional microfabrication method, high resolution ink-jet printing of photo mask materials, was used in the lithography process, eliminating the need for clean room facilities. This resulted in numerous benefits such as reduced cost, fast turn around time from thought-to-design-to-fabrication and implementation and an efficient handling of materials. Micron scale features were realized.