Inhibition of ABTS radical cation and soybean lipoxygenase: In vitro biological activities of selected plant extracts

CHED 586

Jannette Gavillán-Suárez, jgavillan@cayey.upr.edu1, Lisandra Santiago-Capeles, lisandra.santiago@gmail.com1, Yeseida Garay-Cotto1, and Yisel Rivera2. (1) Department of Chemistry, University of Puerto Rico at Cayey, 205 Antonio R. Barceló Ave., Cayey, PR 00736, (2) Medical Sciences Campus, Department of Microbiology, University of Puerto Rico, San Juan, PR 00935
Two assays were used to evaluate the in vitro activity of several plant extracts used in folk medicine to treat various diseases. The antioxidant capacity of Costus sp., Rhoeo spathacea, Tapeinochilus ananassae, and Syzygium jambos methanolic extracts was studied using the “Trolox Equivalent Antioxidant Capacity” (TEAC) colorimetric assay. This method uses Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) as a reference standard to which the antioxidants activities of the test samples are referred. To evaluate the anti-inflammatory capacity of the extracts, the inhibition of Soybean 5-Lipoxygenase (5-LOX) activity was studied. The initial reaction rate for the formation of hydroperoxides was followed at 234 nm. Quercetine was used as reference in both assays. Low antioxidant and anti-inflammatory activities (lower than 1.0 mM TEAC and 50% LOX inhibition) at 0.1 mg/mL were determined. The strategy for the in vitro evaluation of plant's extracts and results of the antioxidant and anti-inflammatory activities are presented
 

Undergraduate Research Poster Session: Organic Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007