Kinetic investigation of the reaction of a fluorescent label and fatty acids

CHED 334

Kelly Ann Wilson, acs@carlow.edu1, John T. Williams, william800@duq.edu2, and Mitchell E. Johnson, johnsonm@duq.edu2. (1) Department of Chemistry and Physics, Carlow University, 3333 Fifth Avenue, Pittsburgh, PA 15213, (2) Department of Chemistry and Biochemistry, Duquesne University, 600 Forbes ave, Pittsburgh, PA 15282
A kinetic investigation of the reaction between a fluorescent label and a fatty acid is necessary in order to gain a more thorough understanding of the capillary electorphoretic separation of fatty acids. Reactions involving caproic (C6), lauric (C12), palmitic (C16), and stearic (C18) acids were run while varying the concentrations of acids and the label. Aliquots of reactions were quenched at specific time intervals. The progress of the reaction under pseudo first order conditions was monitored using non-aqueous capillary electrophoresis coupled with near infrared laser induced fluorescence and photon detection. The reaction rates were also examined while varying the temperature at which the reactions were run.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Chemical Education

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007