Membrane self-assembly at structured surfaces

COLL 75

Michael C Howland, mhowland@gmail.com1, Babak Sanii2, Alan W Szmodis3, Andreia Michelle Smith, amismith@ucdavis.edu4, and Atul N. Parikh, anparikh@ucdavis.edu2. (1) Chemical Engineering and Materials Science, University of California, Davis, 3001 Engineering III, Davis, CA 95616, (2) Applied Science, University of California, Davis, 3001 Engineering III, Davis, CA 95616, (3) Biophysics, University of California, Davis, 3001 Engineering III, Davis, CA 95616, (4) Biophysics Graduate Group, University of California, Davis, 3001 Engineering III, Davis, CA 95616
Structured surfaces, displaying pre-patterned variations of interfacial energies template vesicle fusion and lipid spreading mechanisms at the solid-aqueous interface giving rise to unusual fluid-fluid morphologies and phase-separation behaviours that reflect the underlying pattern. Using periodic hydrophilic/hydrophobic substrates, we demonstrate spontaneous coralling of single fluid lipid bilayers from the surrounding fluid, monolayers by a lipid-free transition region. Such juxtaposition of lipid morphologies in single samples from a single vesicular source provide a useful platform for (1) depositing protein patterns; (2) determining leaflet-dependent partitioning preferences of specific molecules in asymmetric bilayers; (3) creating high-density arrays of bilayer-specific membrane functions; and(4) determining interleaflet cooperativity in regulating lateral phase separation processes.
 

Biological Surface Chemistry
8:30 AM-11:50 AM, Monday, March 26, 2007 McCormick Place South -- Room S404A, Level 4, Oral

Division of Colloid & Surface Chemistry

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007