CHED 345 |
| Capillary Electrophoresis (CE) is an efficient means of separation. Unfortunately, the sensitivity of UV/Vis detection in CE is limited by the capillary's small internal diameter. However, stacking of analytes can be achieved when employing electrokinetic injection (EK), decreasing the detection limits. We have employed stacking using a cholate based run buffer in a Micellar Electrokinetic Chromatographic (MEKC) separation. We report the results of parameter studies on the MEKC separation used to achieve the optimal separation parameters for unmodified nucleosides and the oxidative damage marker 8-OH-deoxyguanosine (8-OH-dG). We present the results of reactions involving double stranded calf thymus DNA and ROS separated using MEKC and demonstrate the successful separation and detection of 8-OH-dG. The results from the MEKC separations were employed to the design of a microfluidic device for chemical analysis. We report a design that incorporates a z-shape detection channel, which increases the sensitivity of UV/Vis absorption detection. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |