CHED 887 |
| Rae1 is a gene found in many eukaryotic cells and can be expressed in E. coli. The mechanisms of rae1 are not known, but rae1 is involved in the complex pathways of mitotic spindle formation and mRNA export. Rae1 is also required for spindle assembly. Schizosaccharomyces pombe has over 50 genes associated with human diseases, this includes 23 of which are associated with DNA damage and repair, checkpoints, and the cell cycle leading to obvious cancer research implications. This particular gene was isolated from S. pombe and the cDNA inserted into a plasmid vector. Various plasmids including this rae1 gene were used to transform two different strains of E. coli to replicate and express the plasmid and resulting protein. Purification would allow for structural studies and site-directed mutagenesis would allow the possibility of working out detailed mechanisms. |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |