Mechanism of carbamoyl formation, pyrimidine synthesis and role of methabolic pathways controling nucleotide synthesis

CHED 605

Radoslav S Bozov,, MU-Sofia, NVCC, UCSF, Center for Dental Anesthesiology, 1016 S.Wayne #106, Arlington, VA 22204
The urea cycle is comprised by five enzymes, but also requires other enzymes and mitochondrial amino acid transporters to function fully. The mechanism of carbamoyl phosphate formation (nitrogen and carbon bond formation is not well understood),nitrogen accretion during pregnancy and decreased urea synthsis. In contrast to the liver cells, where glucagon, insulin and corticoids are major regulators of the urea cycle, in nonheapatic cells the urea cycle enzymes are regulated from various pro and antiinflamatory cytokins and other agents. The existing mechanism for nucleotide synthesis involves enzymes that do not increase their concentration in case of increase cell division. Experiments reveal that urea can form small nucleotides. I used enol formation, creation of nucleophiles, condensation and rearrangement of urea and pyruvate and phosphate to form nucleotides, water and regenarating carbamoyl phosphate, entering a new cycle. The role of arginine methabolism becomes highly complex. My synthesis retains the labeled isotopic atoms in the nucleotides synthesis proven by analytical chemistry. However, the mechanism explains further regulation of the proliferative properties of cells, and chemically explainable by predictive resonance pi bond shift and flaw of induced lone electron pairs. It supports the theory for prebiotic nucleotide formation by using molecules synthesized in Miller's experiments. It would support the approach of epigenetics that acetylation needed for gene regulation and methylation of cytosine are closely related to base synthesis. It could be a new approach in the treatment of disorders based on disrupted regulation of methabolic pathways responsible for lose of control such as cancer.