CHED 288 |
| The detection of transfer ribonucleic acids (tRNAs) was determined using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry for its demonstration of accuracy and sensitive mass determination. Total transfer RNAs mixtures were isolated by an organic and solid-phase extraction from yeast Saccharaomyces cerevisiae strain YPH499 grown in YEPD media. Commercial grade yeast tRNA (Ambion Inc.) and YPH499 were digested with endonuclease RNase T1 and A. Signature products were then detected by mass spectrometry and compared to theoretical data. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |