BIOTWednesday, 13 September 2006

5:30 PM-7:30 PM Moscone Center -- Hall D, Poster
Poster Session
Downstream
Organizers:Claire F. Komives
Sanjeev Ahuja
Chia Chi Ho
Suspension mixing studies for production scale hydroxyapatite column packing
Michael A. Winters, Thomas J. Svab, Robert P. Kasprow, Michael J. Kosinski
Robustness of virus removal by Protein A chromatography is independent of media lifetime and mechanism of degradation
Scott Lute, Lenore Norling, Michael Hanson, Qi Chen, Kurt Brorson
Displacer concentration effects in displacement chromatography: Proteomic applications
Steven Taylor Evans, Alexander S. Freed, Amy Manocchi, Steve M. Cramer
Disposable POD depth filter evaluation
Francisco Valles, Megumi Noguchi, Paul Bezy, Adema Arroyo, Brad Wolk, Marty Siwak
Evaluation of a disposable Q-membrane filter in the purification of a monoclonal antibody
Keqiang Shen, Siegfried Rieble, Jay Leversee, Scott Gangloff, Eugene Schaefer
Flexible purification of histidine-tagged and untagged proteins using various metal ions and IMAC Sepharose 6 Fast Flow
Johan Karlsson, Ellinor Ancker, Marianne Albenius, Lars C Andersson, Makonnen Belew
Impact of cell density and viability on primary clarification of mammalian cell broth
Michael J. Iammarino, Joseph Nti-Gyabaah, Martin Chandler, David J. Roush, Kent E. Göklen
Improving the performance of chromatography columns using computational fluid dynamics
Sanjib Das Sharma, L. Srinivasa Mohan, Richard D. LaRoche
J-Tube vs. Dip Tube: A manufacturing study designed to mininize the level of high molecular aggregates in the phenyl eluate for a IgG4 monolclonal antibody
Carl Hitscherich Jr, Kevin Maloney, Ron Corey, Amin Aubjoub, James Weidner
Paper Withdrawn
Precipitation of plasmid DNA by cationic detergents: Interaction with buffer components and the use of modifiers to aid dissolution
Matthew P. Watson, Sangeetha L. Sagar, Jason C. Murphy
Production and pilot scale recovery of bovine chymosin B from safflower seed
W. Brent R. Pollock, Mitch Beazer, Joseph G. Boothe, Rick Keon, Maurice M. Moloney, Kent Shafer, Helen Yang
Protein purification using new high-capacity ion exchange resins
Jon R. Fisher Jr., Martin Deetz, Amie S. Gehris, Boris Polanyer, John J. Maikner, Marlin K. Kinzey
Rapid removal of detergents from protein solutions using polymeric reversed phase resins
Jon R. Fisher Jr., Amie S. Gehris, John J. Maikner
Selective removal of leached protein A, aggregates and host cell proteins from mAbs by multi-modal anion exchange chromatography
Anders Ljunglöf, Eggert Brekkan, Annika Forss, Martin Antti
Sodium butyrate impacts the expression of genes associated with erythropoietin glycosylation
Christopher K. Crowell, Gustavo Grampp, Qiang Qin, Gary N. Rogers, Richard A. Radcliffe, Robert Scheinman
Paper Withdrawn
Paper Withdrawn
A toolbox for efficient release of therapeutic proteins from the periplasm of Pseudomonas fluorescens
Anant Patkar, Mike Chappell, Yuk Chun Chiu, Jeff Allen, Jefry Shields, Subrata Sen
Simulation of an industrial ion exchange step: The use of simulation for optimization
Thomas Budde Hansen, Steffen Kidal
Affinity adsorption of viruses using small peptide ligands
Caryn L Heldt, Patrick V. Gurgel, Ruben G. Carbonell
Analysis of mAb Protein A chromatography peak precipitates and approaches to reduce peak turbidity
Scott A Tobler, Aaron Noyes, Joseph W. Rajewski III, Russell Shpritzer, Jon Coffman, Suresh Vunnum, Brian Kelley
Automation of a multi-dimensional chromatography lab model for the downstream purification of monoclonal antibodies
William Holmes
Purification of ACE inhibitory peptide using synthetic cloned DNA as tandem multimers expressed in Escherichia coli
Md. Fida Hasan, Yoichi Kumada, Masaaki Terashima, Shigeo Katoh
Binding kinetics of immunoglobulins on affinity membranes
Cristiana Boi, Simone Dimartino, Giulio C. Sarti
Practical issues in the industrial use of hydroxyapatite for purification of monoclonal antibodies
Pete Gagnon, Paul Ng, Julia Zhen, Jie He, Cheryl Aberin, Heather Mekosh
Analysis of adsorptive membranes for DNA clearance
William T. Riordan, Mark R. Etzel
Binding of virus, DNA, and protein particles to anion exchange ligands: Kinetic and transport limitations assessed using surface plasmon resonance
William T. Riordan, Mark R. Etzel
Inclusion body purification method increases protein refold efficiency four-fold for Human Aspartase-2
William A. Miller, Bruce F. Bishop, Stephen E. Sobacke, Marc H. Perez, Paul B. Lanter
Intermediate purification of a monoclonal antibody: Productivity maximization based on multivariate data analysis
Mattias Bryntesson, Martin Antti, Carina Engstrand, Maria Murby, Gustav Rodrigo
Mixed-matrix-membrane alternative in bioseparations
M. Omon Herigstad, Patrick V. Gurgel, Ruben G. Carbonell
Optimization of monoclonal antibody production using process simulation and scheduling tools
Charles A. Siletti, Demetri Petrides
Process analytical technology (PAT) applications to unit operations used for the purification of biotech products
A Sharma, Anurag S Rathore, David Chilin
Process demonstration of a new full-scale protein purification process
Akhil Agrawal, Dicky Abraham, Dave Wohlpart
Process modeling in bioprocess development and technology transfer
D. Petrides, Charles A. Siletti
Cation exchange chromatography (CEX) process development
Jonida Basha, Nihal Tugcu, David J Roush, Kent E Goklen
Paper Withdrawn
Comparison of cation exchange resins using a therapeutic antibody: Experimental and theoretical studies
Lyle R. Lash III, Chris J DeMello, Ajoy Velayudhan, Mark Teeters, Chiali Liu
Comparison of pH and protease stability of an alkali stabilized Protein A derivative with recombinant Protein A
Tomas Björkman, Hans J. Johansson
Clustered-charge adsorbents with enhanced protein affinity and selectivity
Sindhu Balan, Ajish SR. Potty, Richard C. Willson
Paper Withdrawn
Protein phase behavior in polymer solutions: Ovalbumin / PEG 8000 / (NH4)2SO4 and ovalbumin / PEG 8000 / NaCl
André C. Dumetz, Eric W. Kaler, Abraham M. Lenhoff

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The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006