ANYL 158 |
| Bacterial whole-cell biosensing systems should be transportable, robust, easy-to-use and sensitive to be effective in field analysis. To that end, we have designed an alternative method of storage and transport that should broaden the field applications of such biosensing systems. We used the sporulating bacterium Bacillus subtilis for the development of these sensing systems, and the generation of spores as a simple, cheap, stable and resistant way of storage and transport. Spores can be germinated to generate metabolically active cells. To validate our hypothesis, we employed a whole-cell luminescent biosensing system based on reporter gene strategies capable of detecting arsenite. The data obtained demonstrated that after either six months of storage or three cycles of sporulation/regermination, the sensing system retained its analytical performance in terms of detection limit, dynamic range and reproducibility, thus demonstrating the feasibility of employing spores as a means of long-term storage and transportation of whole-cell biosensors. |
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Analytical Chemistry |