ANYL 164 |
| Fluorescent dye doped silica nanoparticles (FSNPs) have significant advantages in signal amplification, photostability, and surface modification for various biological applications. FSNPs have been used to detect overexpressed folate receptors, which are typical for diverse cancer cells including breast cancer and mouth cancer. Monodisperse tetramethylrhodamine-dextran doped silica nanoparticles and tris(2,2'-bipyridyl)dichlororuthenium(II)hexahydrate doped silica nanoparticles of 50 nm diameter were synthesized using a water-in-oil microemulsion method in the presence of an ammonium hydroxide catalyst. The amine modified FSNPs were covalently attached to folic acid conjugated to poly(ethylene glycol) to improve their intracellular uptake and ability to target specific cells. Folate conjugated dye doped silica nanoparticles were targeted to various types of cells, human breast cancer (MDA-MB-231) cells and human mouth cancer (KB) cells. Both MDA-MB-231 and KB cells having overexpressed folate receptors were extensively labeled with FSNPs while normal lymphocyte cells were not labeled. |
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |