ANYL 304 |
| We have developed a modular microsystem for ultra-fast DNA Sanger cycling sequencing, which is composed of two modules: (1) a continuous flow thermal cycler (CFTC) chip consisting of a 20 loop spiral microfluidic channel and three well defined temperature zones for DNA denaturation, renaturation and extension; (2) a solid phase reversible immobilization (SPRI) chip composed of an ordered array of microposts, which was used for the purification of the DNA sequencing ladders prior to electrophoretic sorting. The dye-terminator sequencing cocktail was hydrodynamically pumped through the CFTC chip, mixed with the immobilization buffer and eluted from the SPRI chip prior to capillary array electrophoresis separation. A read length of >600 bp could be obtained in a total reaction time of <30 min (15 min for thermocycling and 10-15 min for purification), compared with 3-4 hours processing time in benchtop instruments. Additionally, the whole process does not require manual manipulation and could be configured in a high throughput format. |
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Analytical Approaches: Separation Science
8:30 AM-12:00 PM, Thursday, 14 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |