Reduction of 4-nitrophenol catalyzed by nitroreductase

BIOL 264

Rongji Dai, dairongji@bit.edu.cn1, Jie Chen, N/A1, Bin Tong2, Weiwei Meng, N/A1, and Yulin Deng, deng@bit.edu.cn1. (1) School of Life Science and Technology, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, China, (2) School of Metarials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Amino-phenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio was studied. The similar reducing ratios of 4-nitrophenol were obtained both under aerobic and under anaerobic conditions and the results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol. Nitroreductase is an oxygen-insensitive enzyme. Moreover, the reductive products of 4-nitrophenol were determined by HPLC and MS.
 

Enzymes
4:30 PM-6:30 PM, Wednesday, 13 September 2006 Moscone Center -- Hall D, Poster

Division of Biological Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006