ANYL 248 |
| Measurement and control of environmental parameters is crucial for the optimization of complex bioprocesses. BioProcessors has developed the SimCell™ Automated Management System that allows hundreds of mammalian cell cultures at the submilliliter-scale to be maintained and monitored automatically. Novel scale-down microbioreactor arrays have been designed to both emulate the environment in traditional bench-top bioreactors and allow for the non-invasive monitoring of pH, dissolved CO2 (dCO2), and dissolved oxygen (DO) using optical chemosensors. Total biomass of the cell cultures was measured by optical density. On-line and derived measurements were used with predictive control schemes and mass transfer models of the microbioreactor as the basis for pH and dissolved oxygen control. The SimCell™ sensing and control strategies will be described. Accurate, on-line measurement and control of cell cultures in microbioreactors will be demonstrated, as will the effects of environmental setpoints on culture performance. |
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Analytical Approaches: Sensors
8:30 AM-11:40 AM, Wednesday, 13 September 2006 Moscone Center -- Room 123, Oral
Division of Analytical Chemistry |