ANYL 308 |
| The use of gold nanoparticles (GNPs) in capillary electrophoresis (CE) with thermal lens microscope (TLM) detection was investigated as a highly sensitive detection scheme. The GNP was used as a specific supporting material. Since the surface plasmon resonance absorption of the GNP exhibits a sensitive response toward an environmental change, sensitive detection of non-absorbing species is expected. By using a background solution containing the GNPs in CE-TLM, a successful label-free detection of several amino acids was achieved. A good linear relationship between the peak area of glutamic acid and its concentration was observed. Fundamental characteristics of GNP-CE-TLM will be discussed. In microchip electrophoresis (MCE) using a cycloolefin polymer chip, TLM was also employed as a high-sensitive detection method. A fast chiral separation and protein analysis of rat serum by MCE-TLM were demonstrated in combination with sweeping as an on-line sample concentration method by using electrokinetic chromatography (EKC) modes. |
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Analytical Approaches: Separation Science
8:30 AM-12:00 PM, Thursday, 14 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |