ANYL 321 |
| The control of anabolic steroid abuse in sports is traditionally performed by gas chromatography-mass spectrometry (GC-MS), requiring chemical derivatization steps and lacks sensitivity in detecting certain steroids. A novel approach for rapid screening of anabolic steroids in urine using MALDI-LIT MS is described. Urine was fortified individually and with a complex mixture of testosterone, betamethasone, nandrolone, stanozolol, cyclofenil, clenbuterol and diclofenac. Ionization efficiency and sensitivity were compared between meso-tetrakis(pentafluorophenyl)porphyrin and three other commonly used MALDI matrices. Porphyrin (MW 974.57) was the preferred matrix due to lack of interfering matrix peaks in the lower mass range (m/z 100-700). This is the first time MALDI-LIT MS has been reported for detecting anabolic steroids in urine samples. This novel MALDI-LIT MS method improved the sensitivity, speed and throughput for complex mixture analysis of anabolic steroids in human urine and is therefore a complementary method to GC/MS for doping control. |
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Analytical Approaches: Mass Spectrometry
8:30 AM-11:20 AM, Thursday, 14 September 2006 Moscone Center -- Room 124, Oral
Division of Analytical Chemistry |