Real-time detection of organophosphate using carbon nanotube based field effect transistor

ANYL 301

Yu Lei, ylei@ntu.edu.sg1, Ningyi Liu, liuny@ntu.edu.sg2, Qing Zhang, eqzhang@ntu.edu.sg2, Xianpeng Cai1, Ashok Mulchandani, adani@engr.ucr.edu3, Wilfred Chen4, Changming Li, ecmli@ntu.edu.sg5, and Mary Chan, mbechan@ntu.edu.sg1. (1) Division of Chemical and Biomolecular Engineering and Center of Biotechnology, Nanyang Technological University, Innovation Center, Blk 1, Unit 100, Level 1, 16 Nanyang Drive, Singapore, 637722, Singapore, (2) School of Electrical and Electronic Engineering, Nanyang Technological University, Nanyang Avenue, Singapore, 639798, Singapore, (3) Department of Chemical and Environmental Engineering, University of California at Riverside, Riverside, CA 92521, (4) Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA 92521, (5) Division of Bioengineering, Nanyang Technological University, Singapore, 637722, Singapore
A novel nanobiosensor for real-time detection of organophosphate has been developed utilizing single-walled carbon nanotubes (SWNTs) and organophosphorus hydrolase (OPH). Horizontally aligned SWNTs are assembled to fabricate field effect transistors using the AC dielectrophoresis technique. OPH, immobilized on the SWNTs by non-specific binding, triggers the enzymatic hydrolysis of organophosphates (OPs) such as paraoxon, consequently causing sensitive change in the channel conductance, which was measured and correlated to the concentration of organophosphate. Our results indicate the novel nanobiosensor has the potential to serve as a simple and universal platform for real-time biosensing.
 

Analytical Approaches: Sensors
8:30 AM-11:50 AM, Thursday, 14 September 2006 Moscone Center -- Room 123, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006