Real-time monitoring of molecular oxygen and induction of photodynamic effects in live C6 Glioma cells using silica-based dynamic nano-platforms

ANYL 172

Wei Tang, wtangz@umich.edu1, Hao Xu2, and Raoul Kopelman, kopelman@umich.edu1. (1) Department of Chemistry, University of Michigan, 930 N. University, Ann arbor, MI 48109, (2) Department of Public Health, University of Michigan, Ann Arbor, MI 48109
Silica-based dynamic nano-platforms for photodynamic therapy (PDT) were prepared and demonstrated here as a means to enable real-time monitoring of oxygen and induction of photodynamic effect in C6 Glioma cells. Approximately 100nm nanoparticles were synthesized using a modified Stöber method. A cell penetrating TAT peptides, which facilitate intracellular delivery, were attached to the exterior surface of the particle via a spacer. These silica-based nanparticles incorporate not only a photosensitizer, methylene blue for photodynamic cell killing but also an oxygen-sensitive fluorescent indicator, Octaethylporphine ketone, to measure the oxygen concentration. Furthermore, preliminary in vitro studies were conducted in C6 Glioma cells. While simultaneously measuring the intracellular oxygen concentration, the photodynamic efficiency of the methylene blue loaded nano-platform was assessed. The influence on PDT of differing intracellular oxygen levels, in hyperoxic and hypoxic conditions, was determined.
 

General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006