BIOT 312 |
| Previous studies have demonstrated that cell culture environments can affect the productivity and oligosaccharide machinery of cells producing recombinant proteins and antibodies. These changes may represent direct effects of the new environment on metabolic processes, altered gene expression, or complex interactions between these two mechanisms. We are exploring the relationship between altered genotype and resulting glycoprotein phenotypes in sodium butyrate treated cells. We chose the human cell line HT1080 and the glycoprotein erythropoietin (rHuEPO) as our model to test these relationships. Through oligonucleotide microarray analysis, we have found that sodium butyrate has altered the expression of a subset of enzymes and transporters associated with glycoprotein processing. Additionally, we have carefully characterized the glycoprotein structure of secreted erythropoietin before and after sodium butyrate treatment. These data will begin, for the first time, to define an altered glycoprotein phenotype through both genomic and functional analysis. It is our belief that a greater mechanistic understanding of the processes linking altered gene expression with enzyme activities will lead to better cellular environments that match glycoprotein productivity with desirable glycoform content. |
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Poster Session
5:30 PM-7:30 PM, Wednesday, 13 September 2006 Moscone Center -- Hall D, Poster
Division of Biochemical Technology |