ANYL 289 |
| Mass spectrometric imaging, a powerful tool in biology and pharmacology, has been widely used to provide information on the chemical identities and spatial distributions of various compounds in biological samples. We describe here a matrix-free imaging method, 2-D SALDI-MS, for small molecule detection on biological surfaces. Comparing to the conventional matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging, this approach offers the benefits of simpler sample preparation, better scanning resolution, and lower background noise in the low mass range. In our proof-of-concept experiments, human cervical cancer cells (HeLa) were used as the biological model. Phosphatidylcholine (PC), one of the major components of the cell membrane, was used as the biomarker to map the location of HeLa cells. The reconstructed ion maps of the PC's headgroup were compared with the optical images. A good correlation of the spatial distribution of cells confirmed the validity of this SALDI-MS imaging approach. The scanning resolution, detection sensitivity, and chemical abundance were compared with MALDI-MS imaging. The potential of using SALDI-MS imaging for small molecule profiling on diseased tissue samples was explored. |
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Analytical Approaches: Mass Spectrometry
1:30 PM-4:20 PM, Wednesday, 13 September 2006 Moscone Center -- Room 124, Oral
Division of Analytical Chemistry |