Antibacterial activities of thin films containing ceragenins

COLL 138

Paul B. Savage, paul_savage@byu.edu, Jason Nielsen, Xinzhong Lai, laixz@chem.byu.edu, Yanshu Feng, Yang Li, Matthew Richard Linford, mrlinford@chem.byu.edu, and Carl Genberg. Department of Chemistry and Biochemistry, Brigham Young University, C100 BNSN, Provo, UT 84602
Ceragenins are steroid-based mimics of antimicrobial peptides, and they are highly active against Gram-negative and positive bacteria. Because the ceragenins preferentially disrupt prokaryotic membranes, it was anticipated that these antimicrobial agents would retain activity while appended to a polymer and immobilized on a surface. Biofilm formation on medical implants is a primary cause of hospital-acquired infections, and antimicrobial surfaces may provide a means of preventing biofilm formation. Ceragenin CSA-13 has been covalently attached to a variety of polymers including polyacroylchloride and polyurethane. The weight percent of CSA-13 in the polymers has been varied with percentages as high as 16%. With lower molecular weight polyurethane polymers, the conjugate retains a small degree of water solubility, and the ceragenin-containing polymer elutes slowly from coated materials. The CSA-13 polymer conjugate retains potent antibacterial activity with rapid bactericidal kinetics. Larger molecular weight polymers containing CSA-13 remain in thin films on surfaces and inhibit biofilm formation.