Mitochondrial magnesium imaging

ANYL 330

Hirokazu Komatsu, komatsu@applc.keio.ac.jp1, Yutaka Shindo2, Takeshi Kubota2, Koji Hotta2, Kotaro Oka2, and Koji Suzuki, suzuki@applc.keio.ac.jp1. (1) Department of Applied Chemistry, Keio University, Hiyoshi 3-14-1, Kouhoku-ku, Yokohama, Japan, (2) Department of Biosciences and Informatics, Keio University, Hiyoshi 3-14-1, Kouhoku-ku, Yokohama, Japan
Mitochondria are the organelles responsible for energy production, apoptosis, calcium storage and 'magnesium' storage. Mg2+ plays important roles for the modulation of various cellular functions. We are focusing on the development of imaging probes and systems monitoring the dynamics of Mg2+ at mitochondria, with the goal of the analyzing disorders of mitochondria that cause neural diseases. Rhodamine-based Mg2+ fluorescent probes were designed which localize at mitochondria. KMG-301 has a charged beta diketone Mg2+ selective binding site and rhodamine as a fluorophore in order to localize mitochondria. KMG-301 showed successful Mg2+ specific fluorescent increase and mitochondrial localization. Further, intracellular Mg2+ and mitochondrial Mg2+ change with FCCP were simultaneously imaged by double staining with KMG-104 and KMG-301. Here we developed novel rhodamine-based KMG-301 and developed a Mg2+ imaging system inside and outside of mitochondria. The chemical biology of the mitochondrial Mg2+ will be clarified by the developed imaging system.
 

Analytical Approaches: Sensors
1:30 PM-4:20 PM, Thursday, 14 September 2006 Moscone Center -- Room 123, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006