Detection of multiple DNA adducts in human lung and esophagus tissue by liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS)

TOXI 56

Robert A. Kanaly, kanaly@eden.env.kyoto-u.ac.jp1, Saburo Matsui1, Tomoyuki Hanaoka2, and Tomonari Matsuda1. (1) Department of Technology and Ecology, Kyoto University, Kyoto, 606-8501, Japan, (2) Epidemiology and Prevention Division, National Cancer Center Research Institute, Tokyo, 104-0045, Japan
The development of strategies designed to detect inter-tissue variations in susceptibility to DNA damage may advance our understanding of the role of DNA adducts in cancer etiology and as biomarkers to exposure. An LC/ESI-MS/MS DNA adduct detection method designed to detect the neutral loss of 2'-deoxyribose from positively ionized 2'-deoxynucleoside adducts transmitting the [M + H]+ > [M + H – 116]+ transition over a total of 374 transitions was applied to analyze esophageal, and peripherally- and centrally-located lung tissue DNA taken from the same individual. The final analysis indicated that the largest adducts were distributed similarly across the samples (84% to 90% similarity; n = 50) but also revealed that there were large differences in the relative amounts of some adducts detected in both lung and esophagus tissue DNA. Adduct identity confirmation by comparison with authentic adduct standards was also performed where possible. The potential of the method is discussed.
 

Poster Presentations and Awards
6:00 PM-10:00 PM, Tuesday, 12 September 2006 Moscone Center -- Room 104, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Sci-Mix

Division of Chemical Toxicology

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006