Analysis of complex biological samples using microfluidic separation techniques, mass spectrometry and multivariate data analysis

ANYL 10

Charlotta Turner, Charlotta.Turner@kemi.uu.se, Per Sjöberg, Rolf Danielsson, Marit Andersson, Karin Markides, and Jonas Bergquist. Department of Physical and Analytical Chemistry, Uppsala University, P.O. Box 599, SE-751 24 Uppsala, Sweden
This presentation aims at giving an overview of current state-of the art research going on at the Department of Analytical Chemistry at Uppsala University in Sweden. Our research involves the preparation of biocompatible, deactivated and thermally stable microcolumns that give high selectivity for solutes of interest and minimized adsorption of difficult solutes (like proteins and peptides) in complex biological samples such as urine, blood plasma and human cerebrospinal fluid. New materials and physical structures (e.g. Solgels) are utilized together with novel surfaces based on in-house developed polymer chemistry. Fast multidimensional separation by on-line LC-CE followed by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICRMS) detection has been developed. Finally, we have developed new multivariate data analysis methods utilizing the entire three-dimensional data set generated by for instance CE-MS to enable discrimination between profiles, corresponding to changes in the metabolic fingerprint.