AGFD 72 |
| Oxidative stress is a state characterized by an excess of reactive oxygen species (ROS) in the body. Stress damaged intestinal epithelial cells (gut inflammatory disorder) is associated with many chronic diseases and the mucosal immune responses. Recently, we have developed phosphopeptides (PPPs) from hen egg yolk phosvitin and found PPPs possess a novel antioxidative activity against oxidative stress in human intestinal epithelial cells, Caco-2. However, their biological activity at the cellular level has not yet fully understood. The objective of this study is to evaluate the regulation of glutathione (GSH) biosynthesis-associated enzymes against oxidative stress in Caco-2 cells. Treatment of 1mM H2O2-induced cells with PPPs increased GSH levels and γ-glutamylcysteine synthetase. Furthermore, intracellular catalase, glutathione reductase, and glutathione S-transferase activities were elevated by addition of PPPs. These data indicate that PPPs can up-regulate cellular GSH biosynthesis-associated enzymes activity, which play key roles against tissue oxidative stress in the human intestinal epithelial cells. |
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General Posters
1:00 PM-3:00 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Agricultural & Food Chemistry |